Searching for persistent and bioaccumulative unknown chemicals using an integrated approach involving a modern analytical instrument and bioassay methods, as well as assessing ecological risk.

The numbers of chemicals produced in industries are increasing with the diversification of lifestyles, but only a few anthropogenic chemicals are legally monitored and controlled. Furthermore, likely, the type of potentially toxic substances and their release into the environment are increasing yearly because their metabolites and degradation products, impurities, and intermediate compounds are exempt from legal regulations, leading to adverse effects on biota. We researched the development of novel searching and identification methods, elucidation of environmental levels, bioaccumulation features, ecological risk assessment, and new persistent contaminants, by integrating chromatographic techniques of advanced analytical equipment and in vitro bioassay.